The rich cultural heritage and plant food diversity of the Mekong region face formidable challenges brought about by the negative effects of modernization and by environmental shifts. Among these plant foods, edible flowers are well rooted in rituals and traditions of the region’s local communities. Unfortunately, industrial-scale food cultivation practices pose threats to the edible flowers and the traditions in which these are entwined, such as the local culinary, slow food, and cultural practices. Researchers from Thailand, China, Myanmar, Lao PDR, and Vietnam collaborated to explore ways in which they could gather information on and craft conservation strategies for edible flowers. The team was able to identify the importance of edible flowers and proposed multifaceted strategies and actions to protect edible flowers. Fostering research collaboration, the framework developed could generate vital insights into long-term industrial development, such as value-adding applications. Global partnerships and effective policy dissemination were also found to be essential to protect edible flower diversity. The framework’s comprehensive approach, centered on community engagement, aims to balance cultural heritage preservation with environmental sustainability, ultimately safeguarding Mekong’s unique botanical and cultural identity.